How RPA Affects the Action of APOBEC3G in Yeast
Author Information
Author(s): Lada Artem G., Waisertreiger Irina S.-R., Grabow Corinn E., Prakash Aishwarya, Borgstahl Gloria E. O., Rogozin Igor B., Pavlov Youri I.
Primary Institution: University of Nebraska Medical Center
Hypothesis
RPA protects genomic ssDNA from the activity of AID/APOBEC enzymes.
Conclusion
RPA inhibits the deamination activity and processivity of APOBEC3G, suggesting it plays a protective role for the genome.
Supporting Evidence
- RPA was shown to severely inhibit A3G activity in vitro.
- Mutations induced by A3G in yeast were primarily single nucleotide changes.
- An inverse correlation was found between RPA concentration and the number of deaminations induced by A3G.
Takeaway
RPA is like a shield that stops a protein called APOBEC3G from making too many changes to DNA, which can be harmful.
Methodology
The study used in vitro deamination assays and yeast expression systems to analyze the effects of RPA on A3G activity.
Limitations
The study primarily focuses on yeast models, which may not fully represent human cellular environments.
Statistical Information
P-Value
p<10−7
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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