Expression and Functional Roles of Angiopoietin-2 in Skeletal Muscles
2011

The Role of Angiopoietin-2 in Skeletal Muscle Cells

publication Evidence: moderate

Author Information

Author(s): Mofarrahi Mahroo, Hussain Sabah N. A.

Primary Institution: McGill University Health Centre

Hypothesis

Skeletal muscle cells constitutively express ANGPT2 and its production is significantly induced by pro-inflammatory cytokines and reactive oxygen species.

Conclusion

ANGPT2 promotes skeletal myoblast survival and differentiation, suggesting it plays a positive role in muscle fiber repair.

Supporting Evidence

  • ANGPT2 is constitutively expressed in human and murine primary skeletal myoblasts.
  • ANGPT2 expression increases significantly during myoblast differentiation into myotubes.
  • ANGPT2 expression in skeletal myoblasts is induced by H2O2 but not by TNFα, IL6, and IL1β.
  • ANGPT2 triggers significant increases in survival and differentiation of skeletal myoblasts.

Takeaway

This study found that a protein called ANGPT2 helps muscle cells survive and grow, especially when they are under stress.

Methodology

Primary skeletal myoblasts were isolated from human and mouse muscles, and their responses to ANGPT2 were measured in vitro.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0022882

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