Analysis of VEGF-A Regulated Gene Expression in Endothelial Cells to Identify Genes Linked to Angiogenesis
2011

Identifying Genes Linked to Angiogenesis

publication Evidence: moderate

Author Information

Author(s): Rivera Corban G., Mellberg Sofie, Claesson-Welsh Lena, Bader Joel S., Popel Aleksander S.

Primary Institution: Johns Hopkins University

Hypothesis

We aimed to identify novel and missing angiogenesis annotations and verify their significance to angiogenesis.

Conclusion

The study identified 293 angiogenesis-related proteins and showed differential regulation of key proteins involved in angiogenesis across endothelial cell lines.

Supporting Evidence

  • The study identified 202 angiogenesis-associated proteins through a graph diffusion approach.
  • Statistical analysis showed that newly associated proteins frequently had perturbed gene expression during angiogenesis.
  • The analysis revealed differential regulation of HIF-1α and HIF-2α across endothelial cell lines.

Takeaway

The researchers found new proteins that help blood vessels grow, which could help in treating diseases like cancer and Alzheimer's.

Methodology

The study integrated the human interactome with known angiogenesis-annotated proteins and analyzed gene expression data from three endothelial cell lines.

Potential Biases

The selection of angiogenesis-annotated proteins may introduce inherent bias.

Limitations

The study may be biased towards highly connected proteins due to the nature of the graph diffusion method.

Statistical Information

P-Value

0.04

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024887

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