Role of MSGb5Cer in Mouse Embryo Development
Author Information
Author(s): Sato Ban, Katagiri Yohko U, Miyado Kenji, Okino Nozomu, Ito Makoto, Akutsu Hidenori, Okita Hajime, Umezawa Akihiro, Fujimoto Junichiro, Toshimori Kiyotaka, Kiyokawa Nobutaka
Primary Institution: National Research Institute for Child Health and Development
Hypothesis
MSGb5Cer-enriched lipid rafts are involved in the cytokinesis of mouse preimplantation embryos.
Conclusion
MSGb5Cer-enriched raft membrane domains participate in cytokinesis in cooperation with the cortical actin network and E-cadherin distribution.
Supporting Evidence
- MSGb5Cer localizes at the interfaces between blastomeres in mouse preimplantation embryos.
- Depletion of cholesterol from the cell membrane reduced MSGb5Cer expression and stopped cleavage.
- Extensive accumulation of MSGb5Cer at interfaces suppressed E-cadherin localization and cleavage.
- Disruption of actin polymerization decreased MSGb5Cer accumulation at interfaces.
- E-cadherin is required for the proper localization of MSGb5Cer at the interfaces.
Takeaway
MSGb5Cer helps mouse embryos divide properly by working with other proteins and structures in the cell.
Methodology
Live-imaging analysis and immunostaining were used to visualize MSGb5Cer and E-cadherin in mouse preimplantation embryos.
Limitations
The study primarily focuses on the early cleavage stage and may not represent later stages of development.
Participant Demographics
BDF1 mice were used for the experiments.
Digital Object Identifier (DOI)
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