Drug Sensitivity in Childhood Leukaemia
Author Information
Author(s): G.J.L. Kaspers, R. Pieters, C.H. Van Zantwijk, P.A.J.M. De Laat, F.C. De Waal, E.R. Van Wering, A.J.P. Veerman
Primary Institution: Free University Hospital, Dutch Childhood Leukemia Study Group
Hypothesis
In vitro drug sensitivity of leukaemic cells might be influenced by contamination with non-malignant cells and the sample source.
Conclusion
Normal peripheral blood lymphocytes are more resistant to most drugs than childhood leukaemic cells.
Supporting Evidence
- Normal PB lymphocytes were significantly more resistant to all drugs tested, except to Maf.
- Leukaemic BM and PB cells from 38 patients showed no significant differences in sensitivity to any of the drugs.
- In 11 of 12 children, leukaemic BM and PB cells showed comparable drug sensitivity profiles.
Takeaway
This study found that normal blood cells are tougher against drugs than leukaemia cells, which is important for treating cancer.
Methodology
The sensitivity of normal PB lymphocytes and leukaemic cells was assessed using the MTT assay with various cytostatic drugs.
Potential Biases
Potential bias due to sample contamination and the subjective nature of the MTT assay.
Limitations
The study did not evaluate the effects of all drugs and focused on a specific patient population.
Participant Demographics
Children with acute lymphoblastic leukaemia and healthy adult volunteers.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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