Cannabidiol-Loaded Lipid Nanoparticles in Hydrogel for Wound Healing
Author Information
Author(s): Lapmanee Sarawut, Bhubhanil Sakkarin, Charoenphon Natthawut, Inchan Anjaree, Bunwatcharaphansakun Phichaporn, Khongkow Mattaka, Namdee Katawut
Primary Institution: Chulabhorn International College of Medicine, Thammasat University, Thailand
Hypothesis
CBD/LNPs enhance therapeutic efficacy when delivered via PVA/SA hydrogels for chronic wound treatment.
Conclusion
CBD/LNP-PVA/SA hydrogel scaffolds significantly accelerate wound healing compared to control groups.
Supporting Evidence
- CBD/LNPs showed enhanced stability and reduced cytotoxicity compared to unencapsulated CBD.
- In vitro assays demonstrated that CBD/LNPs significantly promoted fibroblast migration.
- In vivo experiments revealed that CBD/LNP-PVA/SA hydrogel scaffold significantly accelerated wound closure.
- Histological analysis confirmed improvements in skin morphology in wounds treated with CBD/LNP-PVA/SA hydrogel scaffold.
- Treatment with CBD/LNPs resulted in a 35% increase in the wound closure rate by day 28.
- CBD/LNPs exhibited significant antioxidant activity in dermal fibroblast cells.
- The swelling behavior of PVA/SA hydrogel scaffold was significantly influenced by the concentration of sodium alginate.
- CBD/LNPs maintained cell viability above 80% at concentrations up to 50 ppm.
Takeaway
This study shows that a special gel with CBD helps wounds heal faster by making skin cells move better.
Methodology
The study involved creating CBD-loaded lipid nanoparticles and incorporating them into a PVA/SA hydrogel, followed by in vitro and in vivo testing for wound healing.
Potential Biases
Potential bias in the selection of animal models and lack of cytokine analysis.
Limitations
The study only used male rats, limiting the evaluation of sex differences in wound healing.
Participant Demographics
Adult male Wistar rats, aged 8 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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