Quinolone Resistance in Enterobacteriaceae in Central African Republic
Author Information
Author(s): Frank Thierry, Mbecko Jean R, Misatou Pembe, Monchy Didier
Primary Institution: Institut Pasteur de Bangui
Hypothesis
What are the genetic mechanisms of quinolone resistance among extended-spectrum beta-lactamase-producing Enterobacteriaceae strains in the Central African Republic?
Conclusion
The study found that quinolone resistance in Enterobacteriaceae may be due to a mutation in topoisomerase or the acquisition of a plasmid-borne qnrA1.
Supporting Evidence
- Nine out of seventeen ESBL-producing Enterobacteriaceae were resistant to ciprofloxacin.
- All CTX-M-producing strains were resistant to the tested quinolones.
- All isolates had the same nucleotide mutation at codon 83 of gyrA.
Takeaway
This study shows that some bacteria in the Central African Republic are becoming resistant to important antibiotics, which can make infections harder to treat.
Methodology
Seventeen ESBL-producing Enterobacteriaceae were isolated and tested for antibiotic susceptibility using disc diffusion and agar dilution methods, and genetic analysis was performed using PCR.
Limitations
The study did not determine the MIC values for two qnrA-positive E. coli due to exceeding the highest concentrations tested.
Participant Demographics
The strains were isolated from urine, pus, or stool samples of patients, including both community and hospitalized patients.
Digital Object Identifier (DOI)
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