Effects of Diet and Insulin on Glucose Uptake in Mice
Author Information
Author(s): Kreissl Michael C, Stout David B, Wong Koon-Pong, Wu Hsiao-Ming, Caglayan Evren, Ladno Waldemar, Zhang Xiaoli, Prior John O, Reiners Christoph, Huang Sung-Cheng, Schelbert Heinrich R
Primary Institution: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
Hypothesis
How do dietary state and insulin affect glucose kinetics in the heart, muscles, and brain of mice?
Conclusion
Dietary changes and insulin administration significantly affect glucose metabolism in the heart and skeletal muscle of mice.
Supporting Evidence
- Insulin increased glucose uptake in non-fasted mice by 47%.
- Fasting reduced glucose metabolic rates in the heart and skeletal muscle.
- Dynamic imaging with kinetic modeling is necessary to assess insulin's effect on glucose metabolism.
Takeaway
This study shows that what mice eat and whether they get insulin can change how their bodies use sugar.
Methodology
Mice were divided into four groups based on dietary state and insulin treatment, then imaged using small-animal PET after FDG injection.
Potential Biases
Isoflurane anesthesia may have influenced glucose uptake results.
Limitations
The study did not correct for spillover of activity between arterial blood and myocardium, and the lumped constant was assumed to be constant across groups.
Participant Demographics
Twenty-three male C57BL/6 mice, aged 12-24 weeks.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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