Tollip Is a Mediator of Protein Sumoylation
2009

Tollip and Its Role in Protein Sumoylation

publication Evidence: moderate

Author Information

Author(s): Ciarrocchi Alessia, D'Angelo Romina, Cordiglieri Chiara, Rispoli Ada, Santi Spartaco, Riccio Massimo, Carone Simona, Mancia Anna Laura, Paci Simone, Cipollini Elena, Ambrosetti Davide, Melli Marialuisa

Primary Institution: Department of Biology, Bologna University, Bologna, Italy

Hypothesis

Tollip is involved in the sumoylation process and interacts with sumoylation enzymes.

Conclusion

Tollip is shown to be involved in the sumoylation of proteins, including the IL-1 receptor.

Supporting Evidence

  • Tollip interacts with sumoylation enzymes, suggesting its role in the sumoylation process.
  • Tollip is shown to be sumoylated and may act as a ligase.
  • The IL-1 receptor is identified as a sumoylation target of Tollip.
  • Tollip's nuclear localization suggests a role in nuclear processes.
  • Tollip's interaction with Daxx indicates a potential role in transcriptional regulation.

Takeaway

Tollip helps attach a special tag to proteins that can change how they work in the cell.

Methodology

The study used yeast two-hybrid screening and various cell transfection experiments to analyze Tollip's interactions and functions.

Digital Object Identifier (DOI)

10.1371/journal.pone.0004404

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