Chick Hairy1 protein interacts with Sap18, a component of the Sin3/HDAC transcriptional repressor complex
2007
Chick Hairy1 Protein Interacts with Sap18
publication
Evidence: moderate
Author Information
Author(s): Sheeba Caroline J, Palmeirim Isabel, Andrade Raquel P
Primary Institution: Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal
Hypothesis
How does the Hairy1 protein interact with other proteins during somitogenesis?
Conclusion
Hairy1 may mediate gene transcriptional repression by recruiting the Sin3/HDAC complex through its interaction with Sap18.
Supporting Evidence
- Hairy1 protein was found to interact with Sap18 in a yeast two-hybrid assay.
- The interaction was confirmed by co-immunoprecipitation experiments.
- Expression patterns of sap18 and sin3a co-localize with hairy1 during chick embryo development.
Takeaway
This study found that a protein called Hairy1 works with another protein, Sap18, to help control gene activity during the development of chick embryos.
Methodology
Yeast two-hybrid system and co-immunoprecipitation assays were used to identify and confirm the interaction between Hairy1 and Sap18.
Digital Object Identifier (DOI)
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