Offspring of Mothers Fed a High Fat Diet Display Hepatic Cell Cycle Inhibition and Associated Changes in Gene Expression and DNA Methylation
2011

Maternal Obesity and Hepatic Function in Offspring

Sample size: 16 publication 10 minutes Evidence: moderate

Author Information

Author(s): Dudley Kevin J., Sloboda Deborah M., Connor Kristin L., Beltrand Jacques, Vickers Mark H.

Primary Institution: Liggins Institute and the National Research Centre for Growth and Development, University of Auckland, Auckland, New Zealand

Hypothesis

A maternal high fat diet leads to altered regulation of liver development in offspring.

Conclusion

Offspring born to mothers fed a high fat diet show early signs of hepatic dysfunction and are likely predisposed to long-term metabolic issues.

Supporting Evidence

  • Offspring of mothers on a high fat diet were smaller at birth and showed signs of metabolic syndrome.
  • Significant G0/G1 arrest was observed in the livers of offspring born to high fat diet mothers.
  • Altered DNA methylation patterns were found in the Cdkn1a gene in the livers of these offspring.

Takeaway

If a mother eats a lot of fat while pregnant, her babies might have problems with their liver that can make them sick later in life.

Methodology

Livers were collected from male offspring of control and high fat diet mothers at postnatal days 2 and 27, and cell cycle dynamics were measured by flow cytometry.

Limitations

The study used a mixed cell population from whole liver homogenates, which may mask cell-type specific effects.

Participant Demographics

Male offspring of Wistar rats.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0021662

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