Hypericum triquetrifolium—Derived Factors Downregulate the Production Levels of LPS-Induced Nitric Oxide and Tumor Necrosis Factor-α in THP-1 Cells
2011

Effects of Hypericum triquetrifolium on Inflammation

publication Evidence: moderate

Author Information

Author(s): Saad Bashar, AbouAtta Bernadette Soudah, Basha Walid, Hmade Alaa, Kmail Abdalsalam, Khasib Said, Said Omar

Primary Institution: Research and Development Regional Center—The Galilee Society

Hypothesis

This study aims to examine the anti-inflammatory mechanism of Hypericum triquetrifolium by measuring the expression and release of pro-inflammatory cytokines in human monocytic cells.

Conclusion

Hypericum triquetrifolium extracts inhibit the production of LPS-induced TNF-α and nitric oxide by downregulating their gene expressions.

Supporting Evidence

  • Hypericum triquetrifolium extracts significantly suppressed the LPS-induced nitric oxide release.
  • The extracts inhibited the transcription of iNOS and TNF-α in a dose-dependent manner.
  • No significant effects were observed on the release of IL-6.

Takeaway

This study found that a plant called Hypericum triquetrifolium can help reduce inflammation in cells by lowering certain harmful substances.

Methodology

The study used THP-1 human monocytic cells treated with lipopolysaccharide and varying concentrations of Hypericum triquetrifolium extracts to measure levels of nitric oxide and pro-inflammatory cytokines.

Limitations

The study was conducted in vitro, which may not fully represent in vivo effects.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1093/ecam/nen056

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