Altered Expression of Somatostatin Receptors in Pancreatic Islets from NOD Mice Cultured at Different Glucose Concentrations In Vitro and in Islets Transplanted to Diabetic NOD Mice In Vivo
2011

Changes in Somatostatin Receptors in Diabetic Mice

Sample size: 12 publication 10 minutes Evidence: moderate

Author Information

Author(s): Ludvigsen Eva, Stridsberg Mats, Janson Eva T., Sandler Stellan

Primary Institution: Uppsala University

Hypothesis

Are the changes in pancreatic islet somatostatin receptor expression in diabetic NOD mice due to hyperglycemia or immune reactions?

Conclusion

The study suggests that hyperglycemia alone does not explain the changes in somatostatin receptor expression in diabetic NOD mice.

Supporting Evidence

  • Diabetic NOD mice showed higher somatostatin receptor expression compared to normoglycemic mice.
  • Islets cultured at high glucose concentrations exhibited more changes in receptor expression than those transplanted in vivo.
  • Beta and PP cells showed more dynamic changes in receptor expression under high glucose conditions.

Takeaway

This study looked at how sugar levels affect certain receptors in the pancreas of diabetic mice, finding that sugar alone isn't the only reason for changes in these receptors.

Methodology

NOD mice islets were cultured at different glucose concentrations and transplanted into diabetic and normoglycemic mice to evaluate somatostatin receptor expression.

Potential Biases

Potential bias due to the small sample size and the specific conditions of the in vitro and in vivo environments.

Limitations

The number of implanted islets was insufficient to cure hyperglycemia in diabetic mice.

Participant Demographics

Inbred NOD mice, both diabetic and normoglycemic, aged 4-7 weeks.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1155/2011/623472

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