HGF and Nucleolin in Prostate Cancer Cell Adhesion
Author Information
Author(s): Tate Amanda, Isotani Shuji, Bradley Michael J, Sikes Robert A, Davis Rodney, Chung Leland WK, Edlund Magnus
Primary Institution: Emory University School of Medicine
Hypothesis
Can nucleolin function as a novel receptor for Hepatocyte Growth Factor (HGF) in prostate cancer cells that lack the Met receptor?
Conclusion
Nucleolin may act as a novel receptor for HGF, influencing cell adhesion in prostate cancer cells even in the absence of the Met receptor.
Supporting Evidence
- HGF was identified as a major signaling component in prostate stromal-conditioned media.
- Nucleolin was found to increase on the cell surface during cancer progression.
- Antibodies against nucleolin blocked the adhesion-stimulatory effects of HGF.
- C4-2 cells showed a concentration-dependent response to HGF.
- Cell spreading was enhanced on laminin-1 substrates in response to HGF.
Takeaway
This study found that a protein called nucleolin helps prostate cancer cells stick together when they are exposed to a growth factor called HGF, even when they don't have the usual receptor for it.
Methodology
The study used mass spectrometry to identify nucleolin as an HGF binding partner and assessed its role in cell adhesion through various assays.
Limitations
The study primarily focused on in vitro models, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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