Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System
Author Information
Author(s): Shilova Nadezhda, Nokel Alexey, Lipatnikov Alexander, Khasbiullina Nailya, Knirel Yuri, Baidakova Ludmila, Tuzikov Alexander, Khaidukov Sergei, Obukhova Polina, Henry Stephen, Shoibonov Batozhab, Salimov Emin, Rieben Robert, Bovin Nicolai, Collin Mattias, Vidarsson Gestur
Primary Institution: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science
Hypothesis
Do all human anti-glycan antibodies activate the complement system?
Conclusion
About 30% of human anti-glycan antibodies do not activate the complement system.
Supporting Evidence
- 30% of anti-glycan antibodies did not activate the complement system.
- Most non-activating antibodies were IgGs directed to bacterial O-antigens.
- Complement-activating antibodies were confirmed through hemolysis tests.
- Printed glycan arrays were used to analyze antibody specificity.
- Antibodies to blood group systems were among those that activated complement.
Takeaway
Some antibodies in our blood can recognize germs but don't always trigger the body's defense system to fight them.
Methodology
The study used a printed glycan array to analyze the ability of human blood antibodies to activate the complement system.
Potential Biases
Potential bias in the selection of serum samples from healthy donors.
Limitations
The study only examined a limited number of donors and specific antibody types.
Participant Demographics
Five healthy adult blood donors, including both males and females, aged 31 to 56.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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