Isolation of novel coregulatory protein networks associated with DNA-bound estrogen receptor alpha
2008
Isolating Protein Networks Linked to Estrogen Receptor Alpha
publication
Evidence: moderate
Author Information
Author(s): Jennifer R Schultz-Norton, Yvonne S Ziegler, Varsha S Likhite, John R Yates, Ann M Nardulli
Primary Institution: University of Illinois at Urbana-Champaign
Hypothesis
The study aims to develop a method for isolating proteins associated with DNA-bound estrogen receptor alpha (ERα).
Conclusion
The developed agarose gel fractionation method is effective for identifying proteins that interact with DNA-bound transcription factors.
Supporting Evidence
- The method allows for the identification of interconnected networks of proteins that influence estrogen-responsive gene expression.
- Mass spectrometry was used to analyze the proteins associated with the DNA-bound ERα.
- The study highlights the complexity of protein interactions involved in regulating gene expression.
Takeaway
The researchers created a new way to find proteins that work with estrogen receptors, which help control how genes respond to estrogen.
Methodology
The study used agarose gel fractionation to isolate protein-DNA complexes involving ERα and HeLa nuclear extracts.
Digital Object Identifier (DOI)
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