Molecular Markers for Early Detection of Cervical Cancer
Author Information
Author(s): Cheng Q, Lau W M, Chew S H, Ho T H, Tay S K, Hui K M
Primary Institution: National Cancer Centre, Singapore
Hypothesis
To identify novel cellular genes that could potentially act as predictive molecular markers for human cervical cancer.
Conclusion
The study identified several genes, including the ribosomal protein S12 gene, that are over-expressed in cervical cancer and could serve as early diagnostic markers.
Supporting Evidence
- Twenty-eight cDNA clones were isolated that were over-expressed in squamous cell cervical cancer biopsies.
- Expression of the ribosomal protein S12 gene was detected in normal tissues adjacent to cancer lesions.
- Some genes were upregulated in early FIGO stages of cervical cancer.
Takeaway
Researchers found specific genes that can help detect cervical cancer early, which could lead to better treatment options.
Methodology
The study used RT–PCR differential display and Northern blot analysis to compare gene expression profiles between cancerous and normal tissues.
Potential Biases
Potential contamination of normal tissues with tumor cells could affect the results.
Limitations
The study used large tissue biopsies that may contain a mixture of cell types, making it difficult to isolate specific tumor markers.
Participant Demographics
Patients with squamous cell cervical carcinoma of different FIGO stages.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website