Suppression of telomerase reverse transcriptase (hTERT) expression in differentiated HL-60 cells: regulatory mechanisms
1999
How HL-60 Cells Control Telomerase Activity During Differentiation
publication
Evidence: moderate
Author Information
Author(s): Xu D, Gruber A, Björkholm M, Peterson C, Pisa P
Primary Institution: Karolinska Hospital, Stockholm, Sweden
Hypothesis
How is telomerase activity regulated in differentiated HL-60 cells?
Conclusion
The study found that hTERT expression is down-regulated before telomerase activity is suppressed in differentiated HL-60 cells.
Supporting Evidence
- hTERT expression is down-regulated before telomerase activity is suppressed.
- The down-regulation of hTERT is achieved through inhibition of gene transcription.
- New protein synthesis is required for the down-regulation of hTERT expression.
- Serum-deprivation does not significantly reduce hTERT mRNA levels within the first 24 hours.
Takeaway
When certain blood cells mature, they stop making a protein that helps them live forever, which is important for understanding how cells grow and change.
Methodology
The study examined the regulation of hTERT expression and telomerase activity in HL-60 cells during differentiation.
Digital Object Identifier (DOI)
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