Rapid Detection of Tet(X) Resistance Gene in Enterobacteriaceae
Author Information
Author(s): Zhang Liyun, Xie Jie, Qu Ziyu, Duan Duan, Liu Chujun, Zhang Di, Jiang Haiyang, Dai Xinhua, Jiang You, Fang Xiang, Wu Congming
Primary Institution: National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University
Hypothesis
A rapid detection method is needed to monitor the spread of tigecycline resistance.
Conclusion
The LC-MS/MS method for detecting the tet(X) resistance gene showed high sensitivity and specificity, making it suitable for routine use in clinical microbiology laboratories.
Supporting Evidence
- The LC-MS/MS method demonstrated a sensitivity of 98.9% and specificity of 100%.
- The method provides results within 1 hour, making it suitable for routine use.
- 35 tet(X)-positive and 15 tet(X)-negative strains were used to optimize the cutoff value.
- The study established a metabolic ratio threshold of 0.291 to differentiate tet(X) producers.
- Results were validated with 70 bacterial strains, showing high concordance with PCR results.
Takeaway
Scientists created a quick test to find a gene that makes bacteria resistant to a strong antibiotic, helping doctors know how to treat infections better.
Methodology
The study developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to detect the tet(X) gene in bacterial isolates.
Limitations
The study did not test additional variants of the tet(X) gene.
Participant Demographics
The strains included 93 tet(X) producers and 30 non-tet(X) producers from farms and slaughterhouses.
Statistical Information
P-Value
0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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