Method for Analyzing Digoxin and Metildigoxin in Blood
Author Information
Author(s): Paula Melo, Rita Machado, Helena M. Teixeira
Primary Institution: National Institute of Legal Medicine, North Branch
Hypothesis
A sensitive and rapid method for the identification and quantification of digoxin and metildigoxin in whole blood can be developed using UPLC-MS/MS.
Conclusion
The developed UPLC-MS/MS method is specific, sensitive, and suitable for routine forensic analysis of digoxin and metildigoxin in postmortem blood samples.
Supporting Evidence
- The method showed high sensitivity with a limit of detection of 0.09 ng/mL.
- Percentage recovery ranged from 83 to 100% for digoxin and from 62 to 94% for metildigoxin.
- The method was validated according to ICH guidelines for selectivity, linearity, and precision.
Takeaway
This study created a quick and accurate way to test for certain heart medications in blood, which is important for understanding cases of poisoning.
Methodology
The method involved solid-phase extraction followed by UPLC-MS/MS for the analysis of digoxin and metildigoxin in whole blood.
Limitations
The study primarily focused on the analysis of digoxin and metildigoxin, and may not be applicable to other cardiac glycosides.
Digital Object Identifier (DOI)
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