In silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans
2006

Identifying Vaccine Candidates Against Leptospira interrogans

publication Evidence: moderate

Author Information

Author(s): Yang Hong-Liang, Zhu Yong-Zhang, Qin Jin-Hong, He Ping, Jiang Xu-Cheng, Zhao Guo-Ping, Guo Xiao-Kui

Primary Institution: Shanghai Jiao Tong University School of Medicine

Hypothesis

Can we identify conserved protective antigens in Leptospira interrogans that may elicit long-term protection?

Conclusion

A total of 226 genes were identified as potential vaccine candidates against Leptospira interrogans, which are conserved and have high transcriptional levels.

Supporting Evidence

  • 226 genes were identified as potential vaccine candidates using genomic approaches.
  • These genes are conserved among ten epidemic serovars in China.
  • The proteins encoded by these genes are potentially surface-exposed.

Takeaway

Scientists found 226 genes in a bacteria that could help make a better vaccine against a disease called leptospirosis.

Methodology

Bioinformatics, comparative genomic hybridization (CGH), and transcriptional analysis were used to identify vaccine candidates.

Limitations

Some genes identified have unknown functions or lack COG annotations.

Digital Object Identifier (DOI)

10.1186/1471-2164-7-293

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