Using DNA metabarcoding and direct behavioural observations to identify the diet of proboscis monkeys (Nasalis larvatus) in the Kinabatangan Floodplain, Sabah
2025

Diet of Proboscis Monkeys in the Kinabatangan Floodplain

Sample size: 155 publication 10 minutes Evidence: moderate

Author Information

Author(s): Thiry Valentine, Boom Arthur F., Stark Danica J., Hardy Olivier J., Beudels-Jamar Roseline C., Vercauteren Drubbel Regine, Alsisto Sylvia, Vercauteren Martine, Goossens Benoit

Primary Institution: Université Libre de Bruxelles

Hypothesis

What is the diet composition of proboscis monkeys in the Kinabatangan Floodplain?

Conclusion

The study identified at least 89 different plant taxa consumed by proboscis monkeys, highlighting the importance of various plant species for their diet.

Supporting Evidence

  • The study identified 67 plant species through direct observations.
  • DNA metabarcoding revealed 100 plant taxa in proboscis monkey feces.
  • At least 22 new genera were added to the known diet of proboscis monkeys.
  • Feeding behavior varied significantly between wet and dry seasons.
  • Direct observations provided higher taxonomic resolution than DNA metabarcoding.
  • Proboscis monkeys primarily consumed leaves, with a seasonal increase in fruit consumption.
  • DNA metabarcoding allowed for the identification of plant taxa not easily observed.
  • The study contributes to conservation strategies for the endangered proboscis monkey.

Takeaway

Proboscis monkeys eat a lot of different plants, and scientists used special methods to find out what they like to eat.

Methodology

The study used direct behavioral observations and DNA metabarcoding of fecal samples to analyze the diet of proboscis monkeys.

Potential Biases

Potential overrepresentation of certain plant species due to the nature of DNA recovery from feces.

Limitations

The DNA metabarcoding method may not accurately reflect the proportions of different plant parts consumed.

Participant Demographics

Proboscis monkeys (Nasalis larvatus) in the Lower Kinabatangan Wildlife Sanctuary.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0316752

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