Accuracy of Immunodiagnostic Tests for Active Tuberculosis Using Single and Combined Results: A Multicenter TBNET-Study
2008

Accuracy of Tests for Active Tuberculosis

Sample size: 425 publication 10 minutes Evidence: moderate

Author Information

Author(s): Goletti Delia, Carrara Stefania, Butera Ornella, Amicosante Massimo, Ernst Martin, Sauzullo Ilaria, Vullo Vincenzo, Cirillo Daniela, Borroni Emanuele, Markova Roumiana, Drenska Roumiana, Dominguez José, Latorre Irene, Angeletti Claudio, Navarra Assunta, Petrosillo Nicola, Lauria Francesco Nicola, Ippolito Giuseppe, Migliori Giovanni Battista, Lange Christoph, Girardi Enrico

Primary Institution: National Institute for Infectious Diseases (INMI) L. Spallanzani, IRCCS, Rome, Italy

Hypothesis

Can a novel assay using RD1 selected peptides accurately diagnose active tuberculosis compared to existing tests?

Conclusion

The novel assay has similar accuracy for diagnosing active tuberculosis compared to traditional tests, but combining it with other tests may improve diagnostic accuracy.

Supporting Evidence

  • The novel test showed a sensitivity of 73.1% for active tuberculosis.
  • Combining the novel test with TST increased sensitivity to 92.4%.
  • The specificity of the novel test was 70.6%, higher than TST's 48.0%.
  • Indeterminate results were found in 2.5% of subjects.
  • Patients with confirmed tuberculosis had a median age of 34 years.

Takeaway

This study looked at a new test for tuberculosis and found it works similarly to older tests, but using them together can help doctors be more sure about their diagnosis.

Methodology

Patients with suspected tuberculosis were tested using a novel assay and compared with traditional tests like TST and QuantiFERON.

Potential Biases

Potential bias due to varying testing methods across centers.

Limitations

Not all individuals were tested by all assays in parallel, and the number of immunocompromised patients was limited.

Participant Demographics

{"age":{"median":40},"gender":{"male":262,"female":151},"ethnicity":{"Africa":46,"Asia":36,"Eastern Europe":102,"South America":27,"Western Europe":202}}

Statistical Information

P-Value

p<0.05

Confidence Interval

95% CI, 66.7–78.9%

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003417

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