Hepatitis B Virus Genotypes and Mutations in Karachi
Author Information
Author(s): Abbas Zaigham, Muzaffar Rana, Siddiqui Arif, Naqvi Syed Ali Anwar, Rizvi Syed Adibul Hassan
Primary Institution: Sindh Institute of Urology and Transplantation, Karachi, Pakistan
Hypothesis
The study aims to determine the prevalence of HBV genotypes and mutations in the precore and basal core promoter regions in HBV strains in Karachi.
Conclusion
Type D is the main HBV genotype in Karachi, and many patients have significant mutations in the PC and BCP regions.
Supporting Evidence
- Genotype D was present in 100% of the patients.
- 38% of HBeAg negative patients had no detectable mutations.
- 24% of HBeAg positive patients had mutations in the PC and BCP regions.
- 62% of HBeAg negative patients had mutations in the PC and BCP regions.
Takeaway
This study found that most people with hepatitis B in Karachi have a specific type of the virus and many have changes in their virus that could affect their health.
Methodology
The study enrolled 109 chronic hepatitis B patients and tested their sera for HBV genotypes and mutations using PCR and line-probe assays.
Potential Biases
The study may have bias due to the convenience sampling method used for patient selection.
Limitations
The study used convenience sampling, which may not represent the true prevalence of HBeAg negative disease.
Participant Demographics
The mean age of participants was 32.6 years, with 78% being male.
Statistical Information
P-Value
0.000
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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