How Myc is Regulated by Ash1 and Polycomb in Drosophila
Author Information
Author(s): Goodliffe Julie M, Cole Michael D, Wieschaus Eric
Primary Institution: Princeton University
Hypothesis
How does the Trithorax Group protein Ash1 interact with Polycomb to regulate Myc activity in Drosophila?
Conclusion
The study suggests that Ash1, along with Polycomb and Pho, is necessary to maintain low levels of expression of Myc targets during embryonic development.
Supporting Evidence
- Ash1 is identified as a modifier of dMyc activity in Drosophila.
- RNAi experiments show that depletion of Ash1 leads to increased expression of Myc targets.
- Microarray analysis reveals overlapping targets between Ash1, Polycomb, and Myc.
Takeaway
This research shows that certain proteins work together to control how much Myc, a protein important for growth, can turn on or off other genes in developing fruit flies.
Methodology
The researchers used a genetic assay in Drosophila, RNA interference, and microarray analysis to study the effects of Ash1 and Polycomb on Myc activity.
Limitations
The study primarily focuses on Drosophila, which may limit the generalizability of the findings to other organisms.
Digital Object Identifier (DOI)
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