Effects of IL-6 on Tumor Growth and Survival in Mice
Author Information
Author(s): Y. Ohel, E.R. Podack, K.J. Olsen, Y. Miyahara, K. Miura, H. Saito, Y. Koishihara, Y. Ohsugi, T. Ohira, K. Nishio, N. Saijo
Primary Institution: National Cancer Center Hospital
Hypothesis
Does the transfection of IL-6 cDNA into Lewis lung carcinoma cells affect tumor growth and survival in mice?
Conclusion
Transfection of IL-6 cDNA into Lewis lung carcinoma cells did not alter tumor growth but caused weight loss and reduced survival in mice.
Supporting Evidence
- Survival times for mice with LLC-IL6 cells were significantly shorter than those with untransfected LLC cells.
- Body weight and serum albumin levels were significantly lower in LLC-IL6 transplanted mice.
- Injection of anti-human IL-6 mAb prolonged the survival of LLC-IL6 transplanted mice.
Takeaway
When scientists added a special gene to cancer cells, the mice lost weight and lived shorter lives, even though the tumors didn't grow bigger.
Methodology
Lewis lung carcinoma cells were transfected with IL-6 cDNA and transplanted into C57BL/6 mice to observe survival and weight changes.
Limitations
The study was limited to a specific mouse model and may not fully represent human responses.
Participant Demographics
Inbred 5-week-old female C57BL/6 mice were used.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Want to read the original?
Access the complete publication on the publisher's website