Mapping Genes and Proteins in Tissues
Author Information
Author(s): Fan Rong, Enninful Archibald, Zhang Zhaojun, Klymyshyn Dmytro, Zong Hailing, Bai Zhiliang, Farzad Negin, Su Graham, Baysoy Alev, Nam Jungmin, Yang Mingyu, Lu Yao, Zhang Nancy, Braubach Oliver, Xu Mina, Ma Zongming
Hypothesis
Can combining imaging and sequencing techniques improve our understanding of cellular processes in tissues?
Conclusion
The DBiTplus method allows for detailed mapping of gene expression and protein presence in tissues, enhancing our understanding of cellular functions.
Supporting Evidence
- DBiTplus allows for single-cell resolution in tissue analysis.
- The method integrates transcriptomics and proteomics on the same tissue section.
- It was successfully applied to various tissue types, including mouse embryos and human lymph nodes.
Takeaway
This study shows a new way to look at both genes and proteins in the same tissue, helping scientists understand how cells work better.
Methodology
The study used a new technique called DBiTplus that combines sequencing and imaging to analyze tissues.
Participant Demographics
The study involved mouse embryos and human lymph nodes.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website