Competition between Delta and the Abruptex domain of Notch
2008
Competition between Delta and the Abruptex domain of Notch
publication
Evidence: moderate
Author Information
Author(s): Pei Zifei, Baker Nicholas E
Primary Institution: Albert Einstein College of Medicine
Hypothesis
The Abruptex domain may serve as a barrier to Notch activation by competing for the ligand-binding domain of Notch.
Conclusion
The Abruptex region may serve as a barrier to Notch activation by competing for the ligand-binding domain of Notch.
Supporting Evidence
- The study demonstrated that the Abruptex domain competes with Delta for binding to the ligand-binding domain of Notch.
- Binding assays showed that both Delta and the Abruptex region of Notch exhibited high affinity binding.
- The findings suggest that the interaction of Delta with Notch is significantly enhanced at acidic pH.
Takeaway
This study found that a part of the Notch protein can block its activation by competing with another protein for the same binding spot.
Methodology
In vitro binding assays were used to detect interactions between the ligand-binding region of Drosophila Notch and other EGF-repeat protein regions.
Digital Object Identifier (DOI)
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