Genetic Changes in Breast Ductal Carcinoma In Situ
Author Information
Author(s): Vos C B J, Haar N T ter, Rosenberg C, Peterse J L, Cleton-Jansen A-M, Cornelisse C J, Vijver M J van de
Primary Institution: Leiden University Medical Center
Hypothesis
Genetic alterations on chromosomes 16 and 17 are important features of ductal carcinoma in situ of the breast and are associated with histologic type.
Conclusion
The study found that different genetic changes are linked to the differentiation of ductal carcinoma in situ, suggesting distinct genetic pathways for its development.
Supporting Evidence
- LOH on chromosome 17 was more frequent in poorly differentiated DCIS (70%) compared to well-differentiated DCIS (17%).
- Loss on chromosome 16 was associated with well- and intermediately differentiated DCIS (66%).
- C-erbB2/neu was amplified in 30% of poorly differentiated DCIS.
- Most frequent alterations in poorly differentiated DCIS included gains on 8q and 17q22–24 and deletion on 17p.
- In well-differentiated DCIS, amplification on chromosome 1q and deletion on 16q were found.
Takeaway
Scientists looked at the genes in breast cancer cells and found that different types of cancer cells have different genetic changes.
Methodology
The study used microsatellite analysis, Southern blotting, and comparative genomic hybridization to analyze genetic alterations in DCIS.
Digital Object Identifier (DOI)
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