scTML: a pan-cancer single-cell landscape of multiple mutation types
2025

scTML: A Database for Single-Cell Mutation Landscapes in Cancer

Sample size: 2582633 publication Evidence: high

Author Information

Author(s): Li Haochen, Ma Tianxing, Zhao Zetong, Chen Yixin, Xi Xi, Zhao Xiaofei, Zhou Xiaoxiang, Gao Yibo, Wei Lei, Zhang Xuegong

Primary Institution: Tsinghua University

Hypothesis

There is a lack of a single-cell-level database containing comprehensive mutation information in all types of cancer.

Conclusion

The scTML database provides a critical resource for exploring single-cell mutation landscapes and their associations with phenotypes across various cancers.

Supporting Evidence

  • scTML includes 636,251 SNVs and insertions/deletions, 98,632 gene fusions, 5,784 alternative splicing events, and 4,866 CNVs.
  • The database allows users to explore mutation-phenotype associations at the single-cell level.
  • Users can analyze single-cell-level mutation-phenotype relationships and detect cell subclusters of interest.

Takeaway

Scientists created a new database that helps us understand how different mutations in cancer cells work together and affect the cells' behavior.

Methodology

The study involved collecting and analyzing single-cell transcriptomic data from 74 datasets, detecting various mutation types, and establishing a comprehensive database.

Limitations

The database currently lacks some high-quality datasets without publicly available raw sequencing data.

Digital Object Identifier (DOI)

10.1093/nar/gkae898

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