Identifying Proteins for a Swine Vaccine Against Actinobacillus pleuropneumoniae
Author Information
Author(s): Zhang Wei, Shao Jing, Liu Guangjin, Tang Fang, Lu Yan, Zhai Zhipeng, Wang Yang, Wu Zongfu, Yao Huochun, Lu Chengping
Primary Institution: Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China
Hypothesis
Can immunoproteomic analysis identify novel antigenic proteins from Actinobacillus pleuropneumoniae that elicit an immune response in infected swine and immunized rabbits?
Conclusion
The study identified 42 immunoreactive proteins from the Actinobacillus pleuropneumoniae serotype 1, including several novel candidates for vaccine development.
Supporting Evidence
- The study identified a total of 42 immunoreactive proteins from the APP serotype 1.
- Five immunoreactive protein spots were identified for the first time.
- The proteins included known antigens such as ApxIIA and protective surface antigen D15.
Takeaway
Researchers found new proteins in a bacteria that makes pigs sick, which could help create better vaccines for them.
Methodology
The study used two-dimensional electrophoresis and western blot analysis to identify immunoreactive proteins from bacterial lysates.
Limitations
The genome sequence of APP serotype 1 is incomplete, which may have limited the identification of some proteins.
Participant Demographics
Swine from 29 herds in different provinces of China were used for serum collection.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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