MOUSE SKELETAL MUSCLE OVEREXPRESSION OF TFEB: AN EXERCISE MIMETIC PROMOTING BRAIN HEALTH INDEPENDENT OF AHN
2024

Muscle TFEB and Brain Health

publication

Author Information

Author(s): Hakian Mia, Matthews Ian, Cai Catherine, Cortes Constanza

Primary Institution: University of Southern California

Hypothesis

Overexpression of TFEB in skeletal muscle has the potential to increase adult hippocampal neurogenesis (AHN) in an exercise mimicking model.

Conclusion

The study found no significant difference in AHN levels between TFEB mice and control mice, but indicated that memory function was maintained in the old muscle TFEB mice.

Supporting Evidence

  • Adult hippocampal neurogenesis decreases with age.
  • Voluntary wheel running in mice can reverse the reduction in AHN.
  • TFEB regulates lysosomal and metabolic function.
  • Transgenic mice overexpressing TFEB in skeletal muscle mimic neuroprotective benefits of exercise.

Takeaway

Researchers thought that boosting a protein in muscles could help the brain grow new cells, but it didn't work as expected, although the mice still remembered things well.

Methodology

AHN levels were measured using immunofluorescence in hemibrain cryosections to count immature neurons in the hippocampus.

Participant Demographics

3-month-old control and muscle-TFEB overexpressing mice.

Digital Object Identifier (DOI)

10.1093/geroni/igae098.3676

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