Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography
2007

Quality Control of Concentrated Gegen Tang Granules

Sample size: 14 publication 10 minutes Evidence: high

Author Information

Author(s): Song Jingzheng, Han Quanbin, Qiao Chunfeng, Yip Yuekeung, Xu Hongxi

Primary Institution: Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine

Hypothesis

There was no reliable analytical method available for the quality assessment of GT granules.

Conclusion

A new method using high performance liquid chromatography (HPLC) has been developed for simple and reliable quality control of commercial concentrated GT granules.

Supporting Evidence

  • The regression equations revealed good linear relationships (correlation coefficients: 0.9994–0.9998) between the peak areas and concentrations.
  • The recovery was between 98.8% and 101.7% with good precision and accuracy.
  • Samples from four manufacturers showed similar profiles but contents of the detected markers varied significantly among manufacturers and batches.

Takeaway

Researchers created a new way to check if concentrated herbal medicine called Gegen Tang is good quality, and found that different brands have different amounts of important ingredients.

Methodology

An HPLC method was developed and validated to evaluate the quality of GT granules by analyzing seven marker compounds.

Potential Biases

Potential bias may arise from the selection of manufacturers and batches for analysis.

Limitations

The study did not assess the long-term stability of the method or the effects of different storage conditions on the quality of GT granules.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1749-8546-2-7

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication