Oral tongue cancer gene expression profiling: Identification of novel potential prognosticators by oligonucleotide microarray analysis
2009

Identifying Genes for Oral Tongue Cancer Prognosis

Sample size: 49 publication Evidence: moderate

Author Information

Author(s): Estilo Cherry L, O-charoenrat Pornchai, Talbot Simon, Socci Nicholas D, Carlson Diane L, Ghossein Ronald, Williams Tijaana, Yonekawa Yoshihiro, Ramanathan Yegnanarayana, Boyle Jay O, Kraus Dennis H, Patel Snehal, Shaha Ashok R, Wong Richard J, Huryn Joseph M, Shah Jatin P, Singh Bhuvanesh

Primary Institution: Memorial Sloan-Kettering Cancer Center

Hypothesis

The study aims to identify potential candidate genes as prognostic markers in human oral tongue squamous cell carcinoma (SCC) through gene expression profiling.

Conclusion

The study identified GLUT3 and HSAL2 as significant predictors of prognosis in oral tongue carcinoma.

Supporting Evidence

  • 77 genes were found to be differentially expressed in tongue tumor samples compared to matched normal controls.
  • GLUT3 over-expression correlated with depth of invasion, tumor size, pathological stage, and recurrence.
  • In survival studies, GLUT3 showed prognostic value with disease-free, relapse-free, and overall survival.

Takeaway

Researchers looked at genes in patients with tongue cancer to find out which ones might help predict how well someone will do after treatment.

Methodology

Gene expression profiles were analyzed using Affymetrix HG_U95Av2 arrays and validated with quantitative real-time RT-PCR.

Potential Biases

The heterogeneous nature of the samples may introduce bias.

Limitations

The study had a relatively small sample size and the findings may not be definitive.

Participant Demographics

{"total":49,"age":{"median":59,"range":"35-97"},"gender":{"male":26,"female":23},"smoking_history":{"yes":30,"no":19},"alcohol_history":{"yes":20,"no":26,"unknown":3}}

Statistical Information

P-Value

p<0.0001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2407-9-11

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