Beta-amyloid increases ATBF1 expression linked to neuron death
Author Information
Author(s): Jung Cha-Gyun, Uhm Kyung-Ok, Miura Yutaka, Hosono Takashi, Horike Hirofumi, Khanna Kum Kum, Kim Mi-Jeong, Michikawa Makoto
Primary Institution: National Center for Geriatrics and Gerontology
Hypothesis
Does ATBF1 expression increase in response to beta-amyloid and DNA-damaging drugs in neurons?
Conclusion
The study found that beta-amyloid and DNA-damaging drugs increase ATBF1 expression, which may lead to neuronal death.
Supporting Evidence
- ATBF1 was up-regulated in the brains of Tg2576 mice compared to wild-type mice.
- Aβ1-42 and DNA-damaging drugs increased ATBF1 expression in cultured neurons.
- Knockdown of ATBF1 protected neurons from death induced by Aβ1-42.
- ATM inhibitors blocked ATBF1 function in neuronal death.
- ATBF1 interacts with phosphorylated ATM after treatment with Aβ1-42.
Takeaway
When certain harmful substances are present, a protein called ATBF1 increases in brain cells, which can lead to cell death.
Methodology
The study used Western blot analysis and real-time PCR to measure ATBF1 expression in cultured rat cortical neurons treated with beta-amyloid and DNA-damaging drugs.
Participant Demographics
The study involved Tg2576 mice and primary rat cortical neurons.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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