New Human Bladder Cancer Cell Lines CAL 29 and CAL 185
Author Information
Author(s): Cattan N, Rochet N, Mazeau C, Zanghellini E, Mari B, Chauzy C, Novion H Stora de, Amiel J, Lagrange J-L, Rossi B, Gioanni J
Primary Institution: Laboratoire de Cancérologie, Centre Antoine Lacassagne
Hypothesis
The study investigates the properties of two new human bladder carcinoma cell lines and their responses to growth factors.
Conclusion
The CAL 29 cell line is tumorigenic and undergoes cell scattering and epithelial to mesenchymal transition, while CAL 185 does not exhibit these properties.
Supporting Evidence
- CAL 29 cells were tumorigenic in mice.
- CAL 29 cells underwent cell scattering and EMT when stimulated by growth factors.
- CAL 185 cells did not show tumorigenic properties or EMT.
- Scattering in CAL 29 cells was reversible after growth factor removal.
- Inhibitors of MEK, Src, and PI3-kinase impaired scattering and EMT.
Takeaway
Scientists created two new bladder cancer cell lines to see how they behave, and found that one can grow tumors while the other cannot.
Methodology
The study involved establishing two cell lines from patients and testing their responses to growth factors in vitro.
Participant Demographics
Patients with high-grade bladder tumors.
Digital Object Identifier (DOI)
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