Sequencing Medicago truncatula Genes with 454 Technology
Author Information
Author(s): Cheung Foo, Haas Brian J, Goldberg Susanne MD, May Gregory D, Xiao Yongli, Town Christopher D
Primary Institution: The Institute for Genomic Research
Hypothesis
Does a single 454 Life Science GS20 sequencing run provide new gene discovery from a normalized cDNA library, and are the short reads valuable for gene structure annotation?
Conclusion
The 454 DNA sequencing technology effectively reveals the expression of transcripts from a broad range of gene categories and identifies many rare transcripts in normalized cDNA libraries.
Supporting Evidence
- The sequencing run generated 292,465 reads, which were reduced to 252,384 after cleaning.
- A total of 184,599 unique sequences were generated, with many having no match in existing databases.
- Approximately 30% of the reads produced were not found in the Medicago Gene Index.
Takeaway
This study shows that a special sequencing method can find many new genes in plants, even if the pieces of DNA it reads are short.
Methodology
The study used 454 sequencing technology on a normalized cDNA library to generate and analyze gene sequences.
Limitations
Only a limited portion of each sequence is uncovered, and the study may not capture the entire transcriptome.
Digital Object Identifier (DOI)
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