Detection and Quantification of Begomoviruses in Tomato Plants
Author Information
Author(s): Péréfarres Frédéric, Hoareau Murielle, Chiroleu Frédéric, Reynaud Bernard, Dintinger Jacques, Lett Jean-Michel
Primary Institution: CIRAD, UMR PVBMT CIRAD-Université de la Réunion
Hypothesis
Can a novel synthetic quantification standard improve the detection and quantification of emerging begomoviruses in tomato plants?
Conclusion
Five duplex real-time PCR assays were developed to effectively detect and quantify a wide range of begomoviruses, which could aid in breeding programs and epidemiological surveys.
Supporting Evidence
- Real-time PCRs were optimized for accurate detection and quantification over a range of viral DNA copies.
- Different patterns of viral accumulation were observed between bipartite and monopartite begomoviruses.
- PYMV accumulated more viral DNA than the monopartite viruses at each time point.
Takeaway
Scientists created a new test to find and measure viruses that make tomatoes sick, helping farmers know when to act.
Methodology
Five duplex real-time PCR assays were developed and optimized for detecting and quantifying viral DNA in artificially inoculated tomato plants.
Potential Biases
Potential biases in sampling and DNA extraction processes were minimized using an internal control.
Limitations
The study was limited to artificially inoculated plants and may not fully represent natural infections.
Participant Demographics
Tomato plants were used for the experiments, specifically cv. Farmer.
Statistical Information
P-Value
p<10-8
Confidence Interval
95%
Statistical Significance
p<10-8
Digital Object Identifier (DOI)
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