Analyzing Antibodies for Protein Detection in Other Species
Author Information
Author(s): Hu Yanhui, Gao Chenxi, McKenna William, Xia Baolong, Ariss Majd, Mohr Stephanie, Perrimon Norbert
Primary Institution: Cold Spring Harbor Laboratory
Hypothesis
Can existing anti-phospho-mammalian protein antibodies be useful for detecting phosphorylated proteins in model organisms?
Conclusion
The study found that over 232 sites on 116 Drosophila proteins could potentially be targeted by antibodies developed for mammalian proteins.
Supporting Evidence
- CESA predicts that more than 232 sites on 116 Drosophila proteins can potentially be targeted by the antibodies initially developed at CST.
Takeaway
Scientists think that some antibodies made for studying proteins in humans and other mammals can also help study proteins in fruit flies and other model organisms.
Methodology
The study developed a software tool called CESA to analyze and predict the utility of phospho-specific antibodies in model species.
Digital Object Identifier (DOI)
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