Caffeine Induces Cell Death via Activation of Apoptotic Signal and Inactivation of Survival Signal in Human Osteoblasts
2008
Caffeine Causes Cell Death in Bone Cells
publication
Evidence: moderate
Author Information
Author(s): Lu Pin-Zhen, Lai Ching-Yu, Chan Wen-Hsiung
Primary Institution: Chung Yuan Christian University
Hypothesis
How does caffeine affect cell viability in human osteoblasts?
Conclusion
Caffeine triggers cell death in osteoblasts primarily through apoptosis and causes bone mineral density loss in vivo.
Supporting Evidence
- Caffeine decreases cell viability in osteoblasts in a dose-dependent manner.
- Caffeine induces oxidative stress in osteoblasts.
- Caffeine triggers apoptosis primarily through a mitochondria-dependent pathway.
Takeaway
Caffeine can make bone cells die, which might lead to weaker bones. This happens because caffeine messes with the cells' survival signals.
Methodology
Human osteoblasts were treated with various doses of caffeine, and cell viability was assessed using the MTT assay.
Limitations
The study primarily focuses on in vitro results and may not fully represent in vivo effects.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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