Iron Nanoparticles Increase Endothelial Cell Permeability
Author Information
Author(s): Patrick L Apopa, Yong Qian, Rong Shao, Nancy Lan Guo, Diane Schwegler-Berry, Maricica Pacurari, Dale Porter, Xianglin Shi, Val Vallyathan, Vincent Castranova, Daniel C Flynn
Primary Institution: The Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA
Hypothesis
Does exposure to iron nanoparticles induce an increase in permeability in human microvascular endothelial cells?
Conclusion
Exposure to iron nanoparticles increases endothelial cell permeability through reactive oxygen species production and microtubule remodeling.
Supporting Evidence
- Iron nanoparticles induce an increase in permeability in human microvascular endothelial cells.
- The permeability increase peaked around 30 minutes after exposure.
- ROS production is involved in the regulation of iron nanoparticle-induced microtubule remodeling.
- GSK-3β signaling pathways are important mediators for iron nanoparticle-induced endothelial cell permeability.
Takeaway
Iron nanoparticles can make blood vessel cells more leaky, which might help in delivering medicines better.
Methodology
The study used confocal microscopy, transendothelial electrical resistance measurements, and flow cytometry to analyze the effects of iron nanoparticles on endothelial cells.
Limitations
The study primarily focuses on in vitro results, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website