Neutrophil Dysfunction in Acute Respiratory Distress Syndrome Linked to Interferon-Stimulated Genes
Author Information
Author(s): Malcolm Kenneth C., Kret Jennifer E., Young Robert L., Poch Katie R., Caceres Silvia M., Douglas Ivor S., Coldren Chris D., Burnham Ellen L., Moss Marc, Nick Jerry A.
Primary Institution: National Jewish Health, Denver, Colorado, United States of America
Hypothesis
High ISG expression accompanies a transient reprogramming of the circulating neutrophil phenotype in the context of the inflammatory environment of ARDS, resulting in an impaired ability to kill pathogenic bacteria.
Conclusion
High ISG expression in neutrophils from ARDS patients is associated with reduced ability to kill Staphylococcus aureus and altered inflammatory responses.
Supporting Evidence
- High ISG expression was found in 25 of 95 ARDS patients.
- Neutrophils with high ISG expression showed reduced migration toward interleukin-8.
- High ISG expression was associated with decreased capacity to kill Staphylococcus aureus.
- Neutrophils from ARDS patients with high ISG expression had significantly attenuated p38 MAPk activation.
Takeaway
Some patients with a serious lung condition called ARDS have special genes that make their immune cells not work as well, which can make it harder for them to fight off certain germs.
Methodology
The study involved isolating neutrophils from ARDS patients and analyzing their gene expression and functional responses to bacterial exposure.
Limitations
The study did not identify the source of ISG induction in most patients and did not systematically screen for viral infections.
Participant Demographics
{"age":{"median":52,"range":"21-86"},"gender_distribution":{"males":52.6},"viral_infection_percentage":15.8,"sepsis_or_pneumonia_percentage":77.9,"chronic_dialysis_percentage":4.2,"immuno_compromised_percentage":4.2,"diabetes_percentage":28.4}
Statistical Information
P-Value
9.4×10−15
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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