Five Steps in Leukocyte Extravasation in the Microcirculation by Chemoattractants
Author Information
Author(s): T. Oda, M. Katori, K. Hatanaka, S. Yamashina
Primary Institution: Kitasato University School of Medicine
Hypothesis
The study aims to quantitatively analyze the entire process of the transmigration of PMNs from the bloodstream to the perivascular space at microcirculatory levels.
Conclusion
The study reveals that PMN extravasation involves a complex process with five distinct steps, and that 80-90% of adhering PMNs disappear from the vascular lumen into the venular wall.
Supporting Evidence
- 80-90% of the PMNs that adhered to the venular wall disappeared into it.
- The average time from adhesion to disappearance from the vascular lumen was about 10 minutes.
- PMNs remained in the venular wall for longer than 30 minutes after passing through the endothelial layer.
- The study identified five distinct steps in the process of PMN extravasation.
- Quantitative analysis was performed using a video system to observe PMN behavior.
Takeaway
When white blood cells need to leave the blood and go to where they're needed, they go through five steps, like rolling, sticking, and squeezing through the walls of blood vessels.
Methodology
The study used a video system to analyze PMN extravasation in the microcirculation of hamster cheek pouches, applying chemoattractants and observing the behavior of PMNs.
Limitations
The method used for counting PMNs was not elegant and relied on visual observation from videotapes.
Participant Demographics
Male golden hamsters (Mesocricetus auratus, 120-150 g, 10-16 weeks old) were used in the study.
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