Impaired Cerebrospinal Fluid Lipoprotein-Mediated Cholesterol Delivery to Neurons in Alzheimer's Disease
2024

Cholesterol Delivery Issues in Alzheimer's Disease

publication

Author Information

Author(s): Borràs Carla, Canyelles Marina, Santos David, Rotllan Noemí, Núñez Estefanía, Vázquez Jesús, Maspoch Daniel, Cano-Sarabia Mary, Carmona-Iragui Maria, Sirisi Sònia, Lleó Alberto, Fortea Juan, Alcolea Daniel, Blanco-Vaca Francisco, Escolà-Gil Joan Carles, Tondo Mireia

Hypothesis

The study aimed to examine cholesterol transport via HDL-like particles in cerebrospinal fluid of Alzheimer's disease patients compared to control individuals.

Conclusion

The study found that cerebrospinal fluid HDL-like particles from Alzheimer's disease patients have impaired cholesterol delivery to neurons.

Supporting Evidence

  • Cholesterol efflux from astrocytes to cerebrospinal fluid was similar between Alzheimer's disease patients and controls.
  • Neuronal cholesterol uptake was significantly reduced in the Alzheimer's disease group.
  • 775 proteins associated with HDL-like particles were identified, with 27 proteins differentially expressed.

Takeaway

In Alzheimer's disease, the way cholesterol is delivered to brain cells doesn't work as well, which might be important for understanding the disease.

Methodology

The study evaluated cholesterol transport using radiolabeled unesterified cholesterol and analyzed HDL-like proteome via liquid chromatography-mass spectrometry.

Digital Object Identifier (DOI)

10.21203/rs.3.rs-5682870

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