Effect of MEK Inhibitors on Lung Cancer Cells with Drug Resistance
Author Information
Author(s): Morgillo F, Cascone T, D'Aiuto E, Martinelli E, Troiani T, Saintigny P, De Palma R, Heymach J V, Berrino L, Tuccillo C, Ciardiello F
Primary Institution: Seconda Università degli Studi di Napoli
Hypothesis
The study aims to investigate the molecular mechanisms regulating cancer cell resistance to four different tyrosine kinase inhibitors.
Conclusion
Resistance to four different TKIs is characterized by epithelial to mesenchymal transition (EMT), which is sensitive to MEK inhibitors in human CALU-3 lung adenocarcinoma.
Supporting Evidence
- All four TKI-R CALU-3 cells showed increased invasion, migration and anchorage-independent growth.
- Treatment with MEK inhibitors caused inhibition of cell proliferation and tumor growth in vivo.
- Significant increase in the expression of activated, phosphorylated MET, IGF-1R, AKT, MEK, MAPK and survivin was observed in TKI-R CALU-3 cells.
Takeaway
The study found that lung cancer cells can become resistant to certain drugs, but using MEK inhibitors can help stop their growth.
Methodology
An in vitro model of acquired resistance was developed using the CALU-3 lung adenocarcinoma cell line, with various assays conducted to assess cell proliferation, migration, and invasion.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website