Effects of HDAC Inhibitors on Corneal Stromal Cells
Author Information
Author(s): Zhou Qingjun, Wang Yao, Yang Lingling, Wang Ye, Chen Peng, Wang Yiqiang, Dong Xiaoguang, Xie Lixin
Primary Institution: Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao, China
Hypothesis
Epigenetic regulations may be involved in the differentiation of corneal keratocytes into fibroblasts and myofibroblasts.
Conclusion
HDAC inhibitors may affect corneal stromal cells by inhibiting myofibroblastic differentiation, cell proliferation, migration and by inducing cell senescence.
Supporting Evidence
- HDAC inhibitors suppressed the activation and myofibroblastic differentiation of corneal stromal cells.
- HDAC inhibitors inhibited the proliferation of corneal fibroblasts by decreasing the proportion of cells in S-phase.
- HDAC inhibitors repressed the migration of corneal fibroblasts in a dose-dependent manner.
- HDAC inhibitors induced the senescence state of corneal myofibroblasts.
Takeaway
This study found that certain drugs can stop corneal cells from changing into scar-forming cells and help them stay healthy.
Methodology
Primary human and mouse corneal stromal cells were cultured and treated with HDAC inhibitors, followed by various assays including cDNA microarray, real-time PCR, and western blot analysis.
Limitations
The effects of HDAC inhibitors may be partially reversible.
Statistical Information
Statistical Significance
p<0.05
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