Three Novel Pigmentation Mutants in Mice
Author Information
Author(s): Vicky Tsipouri, John A. Curtin, Pat M. Nolan, Lucie Vizor, Claire A. Parsons, Colin M. Clapham, Ian D. Latham, Lesley J. Rooke, Jo E. Martin, Jo Peters, A. Jackie Hunter, Derek Rogers, Sohaila Rastan, Steve D.M. Brown, Elizabeth M.C. Fisher, Nigel K. Spurr, Ian C. Gray
Primary Institution: GlaxoSmithKline Pharmaceuticals
Hypothesis
Can genome-wide random ENU mutagenesis in mice produce novel pigmentation mutants?
Conclusion
Three novel pigmentation mutants were identified and mapped to specific chromosomal locations.
Supporting Evidence
- Three pigmentation mutants were identified: White toes, Belly spot and white toes, and Dark footpads 2.
- Genome-wide linkage scans were performed to locate the causative mutations.
- Each mutant line showed autosomal dominant inheritance.
Takeaway
Scientists created special mice with different fur colors by changing their genes, and they found out where these changes happened in their DNA.
Methodology
Mice were treated with ENU and then analyzed for pigmentation changes through visual inspection and genetic mapping.
Potential Biases
Potential bias due to genetic background differences between mouse strains.
Limitations
The study may not account for all genetic variations affecting pigmentation.
Participant Demographics
Mice from BALB/c and C3H/HeH strains were used.
Digital Object Identifier (DOI)
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