Inhibition of DNA-dependent protein kinase activity by phosphorothioate oligonucleotides, suramin, and heparin
Author Information
Author(s): Hosoi Y, Matsumoto Y, Tomita M, Enomoto A, Morita A, Sakai K, Umeda N, Zhao H-J, Nakagawa K, Ono T, Suzuki N
Primary Institution: Department of Radiation Oncology, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Hypothesis
The study investigates the effects of phosphorothioate oligonucleotides, suramin, and heparin on DNA-dependent protein kinase activity.
Conclusion
The study found that suramin and phosphorothioate oligonucleotides significantly inhibit DNA-dependent protein kinase activity, potentially sensitizing cells to ionizing radiation.
Supporting Evidence
- Phosphorothioate oligonucleotides inhibit DNA-PK activity in a length-dependent manner.
- Suramin and heparin also inhibit DNA-PK activity with specific IC50 values.
- Treatment with suramin significantly suppressed DNA-PK activity in cultured cells.
Takeaway
This study shows that certain chemicals can stop a protein that helps repair DNA, which might make cancer cells more sensitive to radiation treatment.
Methodology
The study used various cell lines and measured DNA-PK activity in the presence of different concentrations of phosphorothioate oligonucleotides, suramin, and heparin.
Statistical Information
P-Value
0.045
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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