The Ubiquitin-Like Protein PLIC-1 or Ubiquilin 1 Inhibits TLR3-Trif Signaling
2011
PLIC-1 Inhibits TLR3 Activation
publication
Evidence: moderate
Author Information
Author(s): Nabanita Biswas, Shufeng Liu, Ronni Tapani, Steven E. Aussenberg, Weiqun Liu, Takashi Fujita, Tianyi Wang
Primary Institution: University of Pittsburgh
Hypothesis
Does the ubiquitin-like protein PLIC-1 interact with and inhibit the TLR3-Trif signaling pathway?
Conclusion
PLIC-1 is a novel inhibitor of the TLR3-Trif antiviral pathway by reducing the abundance of Trif.
Supporting Evidence
- PLIC-1 interacts with the TIR domain of TLR4.
- Reduction of PLIC-1 enhances TLR3 activation.
- PLIC-1 co-localizes with Trif and the autophagosome marker LC3.
Takeaway
PLIC-1 is a protein that helps control the immune response by stopping a specific signaling pathway that fights viruses.
Methodology
Yeast two-hybrid screening, luciferase reporter assays, co-immunoprecipitation, and confocal microscopy were used to study the interaction and effects of PLIC-1 on TLR3 signaling.
Digital Object Identifier (DOI)
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