Sensitive Test for Gatifloxacin Antibiotic Using Gold-Silver Nanoparticles
Author Information
Author(s): Hendrickson Olga D., Byzova Nadezhda A., Panferov Vasily G., Zvereva Elena A., Xing Shen, Zherdev Anatoly V., Liu Juewen, Lei Hongtao, Dzantiev Boris B.
Primary Institution: A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences
Hypothesis
Can a new method using Au@Ag nanoparticles enhance the sensitivity of detecting gatifloxacin in food samples?
Conclusion
The developed lateral flow immunoassay can detect gatifloxacin at very low concentrations, making it suitable for food safety testing.
Supporting Evidence
- The method achieved a limit of detection of 0.8 pg/mL for gatifloxacin.
- Recovery rates from meat samples ranged from 78.1% to 114.8%.
- The use of Au@Ag nanoparticles significantly enhanced assay sensitivity compared to traditional methods.
Takeaway
This study created a quick test to find a harmful antibiotic in meat using special tiny particles that make the test more sensitive.
Methodology
The study developed an indirect lateral flow immunoassay using gatifloxacin-specific antibodies and Au@Ag nanoparticles for signal enhancement.
Limitations
The method may require further validation with naturally contaminated samples for broader applicability.
Digital Object Identifier (DOI)
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